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◆TOP2A Gene Amplification Detection Probe
1.Fleetness: Tissue probe hybridization time: 2 hours. Cell probe hybridization time: 1 hour.
2.Accuracy: Less non-specific background staining (dyeing). Increase difficult samples detection rate.
3.Reproducibility: Different laboratories test results are highly reproducible.
The TOP2A gene encodes DNA topoisomerase. This enzyme is involved in processes such as chromosome condensation, chromatid separation, and the release of torsional stress that occurs during DNA transcription and replication. The gene encoding this form, TOP2α, is located on chromosome 17, and the β gene is located on chromosome 3. Many mutations in this gene are associated with developmental resistance.
The TOP2A gene amplification probe is labeled with orange dye for TOP2A gene region, and green dye for chromosome 17 centromere region (CEP17). The TOP2A gene tagged region is located at 17q21.2. The CEP17 probe adopts the α-satellite sequence and has a very high specificity. It does not generate hybrids with other chromosome centromeres.
Patients with abnormal TOP2A genes are predictive of a shorter relapse-free survival, and patients with TOP2A gene deletion have worse prognosis. The study of advanced breast cancer found that TOP2A gene anomalies are significantly associated with protein expression and tumor cell sensitivity to anthracyclines. Therefore, the detection of TOP2A gene status has guiding significance for the treatment and prognosis of breast cancer.
Brunello E,et al.(2012) Histopathology 60: 482-8.
Razis E,et al.(2011) Breast Cancer Res Treat 128: 447-56.